Event Information
Toward the Development of Water-Soluble, Aroylhydrazone-Based Ratiometric Iron Sensors
- Abstract:
Iron plays a vital role in numerous biological processes, but an overabundance of cellular iron can be toxic. Components of a ratiometric iron sensor are being developed in order to synthesize a fluorescent sensor capable of monitoring iron concentrations at the subcellular level. This work describes the synthesis and in vitro fluorescence testing of two versions of a proposed ratiometric sensor component based on rhodamine and diethylaminocoumarin fluorophores with a tridentate metal-binding moiety that shows affinity for Fe3+ and Cu2+. These components have led to the synthesis of a first generation ratiometric sensor that exhibits an in vitro ratiometric fluorescence change in response to Fe3+ and Cu2+ in aqueous buffer. Preliminary cell experiments indicate that the sensor is cell-permeable. The further development of this sensor design may result in a sensor capable of imaging changes in intracellular iron concentrations of living cells.
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