Event Information
Synthesis and Characterization of Second- Generation Iron Pro-Chelators
- Abstract:
High levels of loosely bound iron accumulating in tissues or organelles can cause formation of reactive oxygen species through Fenton chemistry. In the presence of cellular reductants, this labile iron can become catalytic, continuously forming hydroxyl radicals from H2O2, which can induce cell death. A set of second-generation iron pro-chelators, which can potentially reveal high affinity metal ligands when oxidized by H2O2, has been described. Unlike traditional iron chelators, which can disrupt iron homeostasis, these pro-chelators have a protective mask that is revealed selectively by H2O2. Preliminary studies on kinetics of oxidation by H2O2, solubility, and stability in various conditions have begun. The most stable pro-chelator in minimal essential media, MEM, was found to be DEA-BSIH. It was the most soluble pro-chelator in water and did not show signs of hydrolysis of the boronic ester mask in sodium phosphate buffer. HAP-INH was the most stable chelator in this study. When exposed to MEM over a 24 h period it showed minimal changes in its UV spectra compared to the others, which show dramatic change after 4 h.
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