Event Information
Characterization of Ramo15, a Type II Thioesterase, From the Ramoplanin Biosynthetic Cluster
- Abstract:
Ramoplanin is a promising antibiotic that kills both methicillin and vancomycin resistant strains of Staphylococcus aureus. Ramoplanin is produced by nonribosomal peptide synthesis through a cluster of enzymes working together to form the polypeptide chain. Several type II thioesterase domain have been shown vital for efficient peptide production, Ramo15 of the ramoplanin biosynthetic cluster is proposed to be one of these thioesterases. To develop a more complete understanding of the role of thioesterase domains in peptide antibiotic biosynthesis, Ramo15 has been expressed, purified, and shown to be active. Additional work to improve assay conditions to obtain kinetic parameters has also been performed.
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