Event Information
Isolation, Expression and Partial Purification of Anhydromuramic Acid Kinase (AnmK) and Efforts Toward Carbohydrate Substrate Synthesis
- Abstract:
Antimicrobial therapy has been an integral part of the fight against bacterial infection; however, antibiotic resistance is becoming increasingly dangerous. Inhibiting the enzymes that control peptidoglycan recycling may hinder bacterial growth. Anhydromuramic acid kinase (AnmK) is a peptidoglycan recycling enzyme that has been isolated, expressed and partially purified in this project. Its function is an exceptionally unique chemical transformation that could elucidate a new family of kinases. Syntheses of the substrate (anhydromuramic acid) and a related carbohydrate (N-acetyl-anhydroglucosamine) are underway for use to probe enzyme function. Future goals of this project include structure-based inhibitor design, x-ray crystallography and mechanistic studies.
Preliminary Examination Seminar
Student Exams Seminar