Event Information
Elucidation of the Ramoplanin Biosynthetic Pathway
- Abstract:
The overall goal of the proposed research is to more fully understand the biosynthesis of the fatty acid N-terminally attached to ramoplanin, a cyclic peptide antibiotic. Several proteins in the biosynthetic pathway were over-expressed and examined for substrate specificity. The assembly of the fatty acid moiety is probed in detail to understand how this functionally required group is biosynthesized and attached to the growing polypeptide chain. Additionally, we will be examining the enzyme responsible for activating two molecules of asparagine in an unconventional method while facilitating the attachment of the fatty acid moiety. This system is unique because its protein composition enables detailed studies of interactions between proteins as well as biochemical and structural characterization of smaller than normal Non-Ribosomal Peptide Synthetase (NRPS) proteins.
Preliminary Examination Seminar
Student Exams Seminar