Event Information
Protein Palmitoylation of Src Oncoproteins
- Abstract:
Src family signaling proteins function at the plasma membrane to control various biological pathways, including those associated with the proliferation of cancer. Localization of these proteins occurs after they are modified with the covalently linked fatty acids myristate and palmitate. Unlike myristate, the enzymology surrounding the attachment of palmitate is not well known. In order to study this process, we propose the development of a new, fluorescence resonance energy transfer (FRET) based assay. The first step towards this goal is to synthesize a lipidated mimic of the N-terminus of Src proteins that incorporates a pair of fluorophores. To date, a variety of approaches including both solid-phase and solution-phase chemistry have been tried. Of these approaches, solution-phase methods show the most promising results.
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