Event Information
The Challenge of Complex Proteomic Samples
- Abstract:
The Challenge of Complex Proteomic Samples: A multiplex analysis method to increase the coverage and number of identified proteins and probe their functional relationships.
The techniques of LC/MS and LC/MS/MS have allowed us to get ever closer to the functional biological systems we are trying to understand. Improvements in both liquid chromatography and in MS technology have increased the number and particularly the dynamic range of the compounds that we can see. The very high-speed data-dependent (MS/MS) analyses currently available have allowed us to see more out of complex samples, to a limit. Presented in this discussion will be a multiplex LC/MS technique that eliminates the under-sampling of data-dependent MS/MS therefore providing much more comprehensive qualitative data as well as relative and absolute quantitative information. While this technique has been successfully adapted to work on many different types of complex mixtures, the greatest challenge has been to characterize peptides from protein digests. The WatersŪ IdentityE High Definition Proteomics? system enables proteomics researchers to reference chromatographic retention time, exact precursor and product ion masses, structural significance, biological context, and plausible detectability of multiple peptides to confidently identify a protein. The IdentityE system incorporates comprehensive peptide ion accounting informatics that visualizes and identifies peptides and proteins with a multi-layered physicochemical model of protein primary structure. The system provides complete and contextually-based information for confident protein identification. The IdentityE system has been applied to identifying and quantifying proteins associated with stroke tolerance in mouse brains.
Host: George Dubay
Departmental Seminar