Abstract:

     The green fluorscence proteins (GFP) are a unique class of proteins involved in bioluminescence of many cnidaria. In the jellyfish Aequorea victoria, the GFP serve as an energy-transfer acceptor receiving energy from an activated photoprotein.  Upon mechanical stimulation of the organism, GFP emits green light spectrally identical to its fluorescence emission.  This highly fluorescent protein is unique due to the nature of the covalently attached chromophore, which is composed of modified amino acid residues within the protein.  In this talk, formation of the Aequoreavictoria GFP chromophore and of variant GFP chromophores as well as their roles in the fluorescence resonance energy transfer system for studying protein-protein interactions will be explored.
 

References:

Adams, S.R.; Campbell, R.; Gross, L.; Martin, B.; Walkup G.; Yao, Y.;Llipis, J.; Tsien, R.,  J.A.C.S. 2002, 124:6063-6076

Brejc, K.; Sixma, T.; Kitts, P.; Kain, S.; Tsien, R.; Ormo, M.; Remington,J., Proc. Natl. Acad. Sci. USA, 1997, 94:2306-2311

Day, R. N.; Periasamy, A.; Schaufele, F.; Methods, 2001,25:4-18

Griffin, B. A.; Adams, S.; Tsien, R., Science, 1998, 281:269-272

Gross, L.; Baird, G.; Hoffman, R.; Baldridge, K.; Tsien, R., Proc.Natl. Acd. Sci. USA , 2000, 97:11990-11995

Kojima, S.; Hirano, T.; Niwa, H.; Ohashi, M.; Inouye, S.; Tsuji! , F.,TetrahedronLett., 1997, 38:2875-878

Ormo, M.; Cubbitt, A.; Kalio, K.; Gross, L.; Tsien, R., Science,1996,272:1392-95

Pollok, B. A.; Heim, R., Trends in Cell Biology, 1999,9:57-60

Romoser V, V.; Hinkle, P.; Persechini, J., J. Biol. Chem., 1997,272:13270-13274

Tsien, R., Nature Biotechnology, 1999, 17:956-957

Tsien, R., Annu. Rev. Biochem. 1998, 67:509-44

Wachter, R.; King, B.; Tsien, R. Boxer, S.; Remington, J., Biochemistry,1997,36:9759-9765